Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

DSpace/Manakin Repository

Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

Citable link to this page

 

 
Title: Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
Author: Freudiger, Christian Wilhelm; Min, Wei; Saar, Brian G.; Lu, Sijia; Holtom, Gary R.; He, Chengwei; Tsai, Jason C.; Kang, Jing Xuan; Xie, Xiaoliang Sunney

Note: Order does not necessarily reflect citation order of authors.

Citation: Freudiger, Christian W., Wei Min, Brian G. Saar, Sijia Lu, Gary R. Holtom, Chengwei He, Jason C. Tsai, Jing X. Kang, and X. Sunney Xie. 2008. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 322(5909): 1857-1861.
Full Text & Related Files:
Abstract: Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis.
Published Version: doi:10.1126/science.1165758
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10875726
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters