Show simple item record

dc.contributor.advisorFranklin, Melissa
dc.contributor.authorBruneaux, Luke Julien
dc.date.accessioned2013-07-25T13:23:09Z
dc.date.issued2013-07-25
dc.date.submitted2013
dc.identifier.citationBruneaux, Luke Julien. 2013. Multiple Unnecessary Protein Sources and Cost to Growth Rate in E.coli. Doctoral dissertation, Harvard University.en_US
dc.identifier.otherhttp://dissertations.umi.com/gsas.harvard:11041en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10880978
dc.description.abstractThe fitness and macromolecular composition of the gram-negative bacterium E.coli are governed by a seemingly insurmountable level of complexity. However, simple phenomenological measures may be found that describe its systems-level response to a variety of inputs. This thesis explores phenomenological approaches providing accurate quantitative descriptions of complex systems in E.coli. Chapter 1 examines the relationship between unnecessary protein production and growth rate in E.coli. It was previously unknown whether the negative effects on growth rate due to multiple unnecessary protein fractions would add linearly or collectively to produce a nonlinear response. Within the regime of this thesis, it appears that the interplay between growth rate and protein is consistent with a non-interacting model. We do not need to account for complex interaction between system components. Appendix A describes a novel technique for real-time measurement of messenger RNA in single living E.coli cells. Using this technique, one may accurately describe the transcriptional response of gene networks in single cells.en_US
dc.description.sponsorshipPhysicsen_US
dc.language.isoen_USen_US
dash.licenseLAA
dc.subjectBiophysicsen_US
dc.subjectE.colien_US
dc.subjectGrowth Lawsen_US
dc.subjectSystem Biologyen_US
dc.subjectUnnecessary Proteinen_US
dc.titleMultiple Unnecessary Protein Sources and Cost to Growth Rate in E.colien_US
dc.typeThesis or Dissertationen_US
dash.depositing.authorBruneaux, Luke Julien
dc.date.available2013-07-25T13:23:09Z
thesis.degree.date2013en_US
thesis.degree.disciplinePhysicsen_US
thesis.degree.grantorHarvard Universityen_US
thesis.degree.leveldoctoralen_US
thesis.degree.namePh.D.en_US
dc.contributor.committeeMemberPrentiss, Maraen_US
dc.contributor.committeeMemberFranklin, Melissaen_US
dc.contributor.committeeMemberFeldman, Garyen_US
dash.contributor.affiliatedBruneaux, Luke Julien


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record