# Monitoring Neural Activity with Bioluminescence during Natural Behavior

 Title: Monitoring Neural Activity with Bioluminescence during Natural Behavior Author: Naumann, Eva Aimable; Kampff, Adam R.; Prober, David A.; Schier, Alexander F; Engert, Florian Note: Order does not necessarily reflect citation order of authors. Citation: Naumann, Eva M., Adam R. Kampff, David A. Prober, Alexander F. Schier, and Florian Engert. 2010. Monitoring neural activity with bioluminescence during natural behavior. Nature Neuroscience 13(4): 513–520. Full Text & Related Files: Naumann et al 2010_FullVersion.pdf (3.002Mb; PDF) Abstract: Existing techniques for monitoring neural activity in awake, freely behaving vertebrates are invasive and difficult to target to genetically identified neurons. We used bioluminescence to non-invasively monitor the activity of genetically specified neurons in freely behaving zebrafish. Transgenic fish with the Ca$$^{2+}$$-sensitive photoprotein green fluorescent protein (GFP)-Aequorin in most neurons generated large and fast bioluminescent signals that were related to neural activity, neuroluminescence, which could be recorded continuously for many days. To test the limits of this technique, we specifically targeted GFP-Aequorin to the hypocretin-positive neurons of the hypothalamus. We found that neuroluminescence generated by this group of ~20 neurons was associated with periods of increased locomotor activity and identified two classes of neural activity corresponding to distinct swim latencies. Our neuroluminescence assay can report, with high temporal resolution and sensitivity, the activity of small subsets of neurons during unrestrained behavior. Published Version: doi:10.1038/nn.2518 Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846983/?tool=pubmed Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11130434 Downloads of this work: