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dc.contributor.authorNaumann, Eva Aimable
dc.contributor.authorKampff, Adam R.
dc.contributor.authorProber, David A.
dc.contributor.authorSchier, Alexander F
dc.contributor.authorEngert, Florian
dc.date.accessioned2013-10-03T15:18:25Z
dc.date.issued2010
dc.identifier.citationNaumann, Eva M., Adam R. Kampff, David A. Prober, Alexander F. Schier, and Florian Engert. 2010. Monitoring neural activity with bioluminescence during natural behavior. Nature Neuroscience 13(4): 513–520.en_US
dc.identifier.issn1097-6256en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11130434
dc.description.abstractExisting techniques for monitoring neural activity in awake, freely behaving vertebrates are invasive and difficult to target to genetically identified neurons. We used bioluminescence to non-invasively monitor the activity of genetically specified neurons in freely behaving zebrafish. Transgenic fish with the Ca\(^{2+}\)-sensitive photoprotein green fluorescent protein (GFP)-Aequorin in most neurons generated large and fast bioluminescent signals that were related to neural activity, neuroluminescence, which could be recorded continuously for many days. To test the limits of this technique, we specifically targeted GFP-Aequorin to the hypocretin-positive neurons of the hypothalamus. We found that neuroluminescence generated by this group of ~20 neurons was associated with periods of increased locomotor activity and identified two classes of neural activity corresponding to distinct swim latencies. Our neuroluminescence assay can report, with high temporal resolution and sensitivity, the activity of small subsets of neurons during unrestrained behavior.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/nn.2518en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846983/?tool=pubmeden_US
dash.licenseOAP
dc.subjectin vivo neural recordingen_US
dc.subjectbehavioren_US
dc.subjectgenetically encoded calcium indicatoren_US
dc.subjectzebrafishen_US
dc.subjectAequorinen_US
dc.titleMonitoring Neural Activity with Bioluminescence during Natural Behavioren_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Neuroscienceen_US
dash.depositing.authorEngert, Florian
dc.date.available2013-10-03T15:18:25Z
dc.identifier.doi10.1038/nn.2518*
dash.contributor.affiliatedNaumann, Eva A.
dash.contributor.affiliatedEngert, Florian
dash.contributor.affiliatedSchier, Alexander


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