Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli

Mosberg, Joshua Adam Weintrob

Citation

Mosberg, Joshua Adam Weintrob. 2013. Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli. Doctoral dissertation, Harvard University.

Abstract

The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale genome engineering projects. However, limited recombination frequency constrains the usefulness of Lambda Red for several important applications. In this work, I utilize a mechanism-guided approach in order to improve the power and utility of Lambda Red recombination.

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