Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli

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Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli

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Title: Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli
Author: Mosberg, Joshua Adam Weintrob
Citation: Mosberg, Joshua Adam Weintrob. 2013. Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli. Doctoral dissertation, Harvard University.
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Abstract: The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale genome engineering projects. However, limited recombination frequency constrains the usefulness of Lambda Red for several important applications. In this work, I utilize a mechanism-guided approach in order to improve the power and utility of Lambda Red recombination.
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156813
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