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dc.contributor.advisorChurch, George McDonald
dc.contributor.authorMosberg, Joshua Adam Weintrob
dc.date.accessioned2013-10-08T20:47:08Z
dash.embargo.terms2014-06-07en_US
dash.embargo.terms2014-06-07
dc.date.issued2013-10-08
dc.date.submitted2013
dc.identifier.citationMosberg, Joshua Adam Weintrob. 2013. Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli. Doctoral dissertation, Harvard University.en_US
dc.identifier.otherhttp://dissertations.umi.com/gsas.harvard:10777en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11156813
dc.description.abstractThe phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale genome engineering projects. However, limited recombination frequency constrains the usefulness of Lambda Red for several important applications. In this work, I utilize a mechanism-guided approach in order to improve the power and utility of Lambda Red recombination.en_US
dc.language.isoen_USen_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectGeneticsen_US
dc.subjectMolecular biologyen_US
dc.subjectLambdaen_US
dc.subjectNucleaseen_US
dc.subjectPrimaseen_US
dc.subjectRecombinationen_US
dc.subjectRecombineeringen_US
dc.subjectReden_US
dc.titleStudying and Improving Lambda Red Recombination for Genome Engineering in Escherichia colien_US
dc.typeThesis or Dissertationen_US
dash.depositing.authorMosberg, Joshua Adam Weintrob
dc.date.available2014-06-07T07:30:53Z
thesis.degree.date2013en_US
thesis.degree.disciplineChemical Biologyen_US
thesis.degree.grantorHarvard Universityen_US
thesis.degree.leveldoctoralen_US
thesis.degree.namePh.D.en_US
dc.contributor.committeeMemberKahne, Danielen_US
dc.contributor.committeeMemberSilver, Pamelaen_US
dc.contributor.committeeMemberAusubel, Fredericken_US
dash.contributor.affiliatedMosberg, Joshua Adam Weintrob


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