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dc.contributor.advisorWeissleder, Ralph
dc.contributor.advisorLanger, Robert S.
dc.contributor.authorShao, Huilin
dc.date.accessioned2013-10-15T13:29:49Z
dash.embargo.terms2015-10-10en_US
dc.date.issued2013-10-15
dc.date.submitted2013
dc.identifier.citationShao, Huilin. 2013. Biosensor Platforms for Molecular Analyses of Circulating Cancer Biomarkers. Doctoral dissertation, Harvard University.en_US
dc.identifier.otherhttp://dissertations.umi.com/gsas.harvard:11134en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11169784
dc.description.abstractSolid cancers often shed (sub)cellular materials into the circulation, such as circulating tumor cells and extracellular microvesicles. Mounting evidence supports that these circulating materials could serve as surrogate cancer markers for classifying primary tumors, stratifying patients for targeted therapies, assessing treatment efficacy, and achieving clinical benefits. A sensor platform capable of sensitive and portable detection of circulating cancer markers would thus be an invaluable tool, that will advance our understanding of tumor biology as well as clinical outcomes. This dissertation describes various systems that we have developed for quantitative analyses of circulating cancer biomarkers. Firstly, we have developed a novel magnetic resonance sensing platform for microvesicle analyses. By using a chip-based platform that combines microfiltration and bioorthogonal nanoparticle targeting, we demonstrate for the first time that magnetic biosensing can be applied for clinical evaluation of circulating microvesicles in blood samples to monitor cancer therapy. Secondly, we have advanced a new plasmonic sensor to achieve label-free detection of microvesicles. Based on periodic nanohole arrays, this platform has been applied for high-throughput protein profiling of microvesicles in native ascites. Finally, we have implemented microfluidic devices to effectively enrich circulating tumor cells from peripheral whole blood, and to enable comprehensive molecular analyses of isolated tumor cells at a single cell resolution. By enabling rapid, sensitive and cost-effective detection of circulating cancer markers, these developed platforms could significantly expand the reach of preclinical and clinical cancer research, in informing therapy selection, rationally directing trials, and improving sequential monitoring to achieve better clinical outcomes.en_US
dc.language.isoen_USen_US
dash.licenseMETA_ONLY
dc.subjectBiophysicsen_US
dc.subjectBiomedical engineeringen_US
dc.subjectBiosensoren_US
dc.subjectCanceren_US
dc.subjectCirculating biomarkersen_US
dc.subjectDiagnosticsen_US
dc.subjectMolecular analysesen_US
dc.subjectPrognosticsen_US
dc.titleBiosensor Platforms for Molecular Analyses of Circulating Cancer Biomarkersen_US
dc.typeThesis or Dissertationen_US
dash.depositing.authorShao, Huilin
dash.embargo.until10000-01-01
thesis.degree.date2013en_US
thesis.degree.disciplineBiophysicsen_US
thesis.degree.grantorHarvard Universityen_US
thesis.degree.leveldoctoralen_US
thesis.degree.namePh.D.en_US
dc.contributor.committeeMemberBreakefield, Xandraen_US
dc.contributor.committeeMemberLee, Hakhoen_US
dc.contributor.committeeMemberWestervelt, Roberten_US
dc.contributor.committeeMemberHogle, Jamesen_US
dash.contributor.affiliatedShao, Huilin


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