dc.contributor.author | Ryu, Sujung | |
dc.contributor.author | Yellen, Gary I. | |
dc.date.accessioned | 2013-10-16T18:29:59Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Ryu, Sujung, and Gary Yellen. 2012. Charge movement in gating-locked HCN channels reveals weak coupling of voltage sensors and gate. The Journal of General Physiology 140(5): 469-479. | en_US |
dc.identifier.issn | 0022-1295 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11179719 | |
dc.description.abstract | HCN (hyperpolarization-activated cyclic nucleotide gated) pacemaker channels have an architecture similar to that of voltage-gated K+ channels, but they open with the opposite voltage dependence. HCN channels use essentially the same positively charged voltage sensors and intracellular activation gates as K+ channels, but apparently these two components are coupled differently. In this study, we examine the energetics of coupling between the voltage sensor and the pore by using cysteine mutant channels for which low concentrations of Cd2+ ions freeze the open–closed gating machinery but still allow the sensors to move. We were able to lock mutant channels either into open or into closed states by the application of Cd2+ and measure the effect on voltage sensor movement. Cd2+ did not immobilize the gating charge, as expected for strict coupling, but rather it produced shifts in the voltage dependence of voltage sensor charge movement, consistent with its effect of confining transitions to either closed or open states. From the magnitude of the Cd2+-induced shifts, we estimate that each voltage sensor produces a roughly three- to sevenfold effect on the open–closed equilibrium, corresponding to a coupling energy of ∼1.3–2 kT per sensor. Such coupling is not only opposite in sign to the coupling in K+ channels, but also much weaker. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | The Rockefeller University Press | en_US |
dc.relation.isversionof | doi:10.1085/jgp.201210850 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483112/pdf/ | en_US |
dash.license | LAA | |
dc.title | Charge movement in gating-locked HCN channels reveals weak coupling of voltage sensors and gate | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | The Journal of General Physiology | en_US |
dash.depositing.author | Yellen, Gary I. | |
dc.date.available | 2013-10-16T18:29:59Z | |
dc.identifier.doi | 10.1085/jgp.201210850 | * |
dash.contributor.affiliated | Yellen, Gary | |