Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β

View/ Open
Author
Hirai, Hideyo
Kamio, Naoka
Huang, Gang
Matsusue, Akiko
Ogino, Shinpei
Kimura, Nobuhiko
Satake, Sakiko
Ashihara, Eishi
Imanishi, Jiro
Maekawa, Taira
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1371/journal.pone.0054862Metadata
Show full item recordCitation
Hirai, Hideyo, Naoka Kamio, Gang Huang, Akiko Matsusue, Shinpei Ogino, Nobuhiko Kimura, Sakiko Satake, et al. 2013. Cyclic AMP responsive element binding proteins are involved in ‘emergency’ granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. PLoS ONE 8(1): e54862.Abstract
In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)–induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559830/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:11180377
Collections
- HMS Scholarly Articles [17875]
Contact administrator regarding this item (to report mistakes or request changes)