The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: the microparticle-associated immune complexes

DSpace/Manakin Repository

The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: the microparticle-associated immune complexes

Citable link to this page

 

 
Title: The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: the microparticle-associated immune complexes
Author: Cloutier, Nathalie; Tan, Sisareuth; Boudreau, Luc H; Cramb, Catriona; Subbaiah, Roopashree; Lahey, Lauren; Albert, Alexandra; Shnayder, Ruslan; Gobezie, Reuben; Nigrovic, Peter Andrija; Farndale, Richard W; Robinson, William H; Brisson, Alain; Lee, David Marvin; Boilard, Eric

Note: Order does not necessarily reflect citation order of authors.

Citation: Cloutier, Nathalie, Sisareuth Tan, Luc H Boudreau, Catriona Cramb, Roopashree Subbaiah, Lauren Lahey, Alexandra Albert, et al. 2013. The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: the microparticle-associated immune complexes. EMBO Molecular Medicine 5(2): 235-249.
Full Text & Related Files:
Abstract: Immunoglobulins, antigens and complement can assemble to form immune complexes (IC). ICs can be detrimental as they propagate inflammation in autoimmune diseases. Like ICs, submicron extracellular vesicles termed microparticles (MP) are present in the synovial fluid from patients affected with autoimmune arthritis. We examined MPs in rheumatoid arthritis (RA) using high sensitivity flow cytometry and electron microscopy. We find that the MPs in RA synovial fluid are highly heterogeneous in size. The observed larger MPs were in fact MP-containing ICs (mpICs) and account for the majority of the detectable ICs. These mpICs frequently express the integrin CD41, consistent with platelet origin. Despite expression of the Fc receptor FcγRIIa by platelet-derived MPs, we find that the mpICs form independently of this receptor. Rather, mpICs display autoantigens vimentin and fibrinogen, and recognition of these targets by anti-citrullinated peptide antibodies contributes to the production of mpICs. Functionally, platelet mpICs are highly pro-inflammatory, eliciting leukotriene production by neutrophils. Taken together, our data suggest a unique role for platelet MPs as autoantigen-expressing elements capable of perpetuating formation of inflammatory ICs.
Published Version: doi:10.1002/emmm.201201846
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569640/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11180390
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters