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dc.contributor.authorTrindade, Anil Julius
dc.contributor.authorMedvetz, Douglas A
dc.contributor.authorNeuman, Nicole A.
dc.contributor.authorMyachina, Faina
dc.contributor.authorYu, Jane J
dc.contributor.authorPriolo, Carmen
dc.contributor.authorHenske, Elizabeth Petri
dc.date.accessioned2013-10-17T14:03:50Z
dc.date.issued2013
dc.identifier.citationTrindade, Anil J., Douglas A. Medvetz, Nicole A. Neuman, Faina Myachina, Jane Yu, Carmen Priolo, and Elizabeth P. Henske. 2013. Microrna-21 is induced by rapamycin in a model of tuberous sclerosis (tsc) and lymphangioleiomyomatosis (lam). PLoS ONE 8(3): e60014.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11180401
dc.description.abstractLymphangioleiomyomatosis (LAM), a multisystem disease of women, is manifest by the proliferation of smooth muscle-like cells in the lung resulting in cystic lung destruction. Women with LAM can also develop renal angiomyolipomas. LAM is caused by mutations in the tuberous sclerosis complex genes (TSC1 or TSC2), resulting in hyperactive mammalian Target of Rapamycin (mTOR) signaling. The mTOR inhibitor, Rapamycin, stabilizes lung function in LAM and decreases the volume of renal angiomyolipomas, but lung function declines and angiomyolipomas regrow when treatment is discontinued, suggesting that factors induced by mTORC1 inhibition may promote the survival of TSC2-deficient cells. Whether microRNA (miRNA, miR) signaling is involved in the response of LAM to mTORC1 inhibition is unknown. We identified Rapamycin-dependent miRNA in LAM patient angiomyolipoma-derived cells using two separate screens. First, we assayed 132 miRNA of known significance to tumor biology. Using a cut-off of >1.5-fold change, 48 microRNA were Rapamycin-induced, while 4 miRs were downregulated. In a second screen encompassing 946 miRNA, 18 miRs were upregulated by Rapamycin, while eight were downregulated. Dysregulation of miRs 29b, 21, 24, 221, 106a and 199a were common to both platforms and were classified as candidate “RapamiRs.” Validation by qRT-PCR confirmed that these microRNA were increased. miR-21, a pro-survival miR, was the most significantly increased by mTOR-inhibition (p<0.01). The regulation of miR-21 by Rapamycin is cell type independent. mTOR inhibition promotes the processing of the miR-21 transcript (pri-miR-21) to a premature form (pre-miR-21). In conclusion, our findings demonstrate that Rapamycin upregulates multiple miRs, including pro-survival miRs, in TSC2-deficient patient-derived cells. The induction of miRs may contribute to the response of LAM and TSC patients to Rapamycin therapy.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0060014en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612076/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectComputational Biologyen_US
dc.subjectGeneticsen_US
dc.subjectGenomicsen_US
dc.subjectImmunologyen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectSignal Transductionen_US
dc.subjectSignaling in Cellular Processesen_US
dc.subjectTor Signalingen_US
dc.subjectMedicineen_US
dc.subjectOncologyen_US
dc.subjectPulmonologyen_US
dc.titleMicroRNA-21 is Induced by Rapamycin in a Model of Tuberous Sclerosis (TSC) and Lymphangioleiomyomatosis (LAM)en_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorHenske, Elizabeth Petri
dc.date.available2013-10-17T14:03:50Z
dc.identifier.doi10.1371/journal.pone.0060014*
dash.contributor.affiliatedTrindade, Anil
dash.contributor.affiliatedMedvetz, Douglas A
dash.contributor.affiliatedYu, Jane J
dash.contributor.affiliatedPriolo, Carmen
dash.contributor.affiliatedHenske, Elizabeth


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