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dc.contributor.authorArakaki, Xianghong
dc.contributor.authorMcCleary, Paige
dc.contributor.authorTechy, Matthew
dc.contributor.authorChiang, Jiarong
dc.contributor.authorKuo, Linus
dc.contributor.authorFonteh, Alfred N
dc.contributor.authorArmstrong, Brian
dc.contributor.authorLevy, Dan
dc.contributor.authorHarrington, Michael G
dc.date.accessioned2013-10-17T14:08:32Z
dc.date.issued2013
dc.identifier.citationArakaki, Xianghong, Paige McCleary, Matthew Techy, Jiarong Chiang, Linus Kuo, Alfred N Fonteh, Brian Armstrong, Dan Levy, and Michael G Harrington. 2013. Na,k-atpase alpha isoforms at the blood-cerebrospinal fluid-trigeminal nerve and blood-retina interfaces in the rat. Fluids and Barriers of the CNS 10: 14.en_US
dc.identifier.issn2045-8118en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11180405
dc.description.abstractBackground: Cerebrospinal fluid (CSF) sodium concentration increases during migraine attacks, and both CSF and vitreous humor sodium increase in the rat migraine model. The Na,K-ATPase is a probable source of these sodium fluxes. Since Na,K-ATPase isoforms have different locations and physiological roles, our objective was to establish which alpha isoforms are present at sites where sodium homeostasis is disrupted. Methods: Specific Na,K-ATPase alpha isoforms were identified in rat tissues by immunohistochemistry at the blood-CSF barrier at the choroid plexus, at the blood-CSF-trigeminal barrier at the meninges, at the blood-retina barrier, and at the blood-aqueous barrier at the ciliary body. Calcitonin gene-related peptide (CGRP), occludin, or von Willibrand factor (vWF) were co-localized with Na,K-ATPase to identify trigeminal nociceptor fibers, tight junctions, and capillary endothelial cells respectively. Results: The Na,K-ATPase alpha-2 isoform is located on capillaries and intensely at nociceptive trigeminal nerve fibers at the meningeal blood-CSF-trigeminal barrier. Alpha-1 and −3 are lightly expressed on the trigeminal nerve fibers but not at capillaries. Alpha-2 is expressed at the blood-retina barriers and, with alpha-1, at the ciliary body blood aqueous barrier. Intense apical membrane alpha-1 was associated with moderate cytoplasmic alpha-2 expression at the choroid plexus blood-CSF barrier. Conclusion: Na,K-ATPase alpha isoforms are present at the meningeal, choroid plexus, and retinal barriers. Alpha-2 predominates at the capillary endothelial cells in the meninges and retinal ganglion cell layer.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/2045-8118-10-14en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636111/pdf/en_US
dash.licenseLAA
dc.subjectCerebrospinal fluid (CSF)en_US
dc.subjectChoroid plexus (CP)en_US
dc.subjectMeningesen_US
dc.subjectRetinaen_US
dc.titleNa,K-ATPase alpha isoforms at the blood-cerebrospinal fluid-trigeminal nerve and blood-retina interfaces in the raten_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalFluids and Barriers of the CNSen_US
dash.depositing.authorLevy, Dan
dc.date.available2013-10-17T14:08:32Z
dc.identifier.doi10.1186/2045-8118-10-14*
dash.contributor.affiliatedLevy, Dan


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