TREX exposes the RNA binding domain of Nxf1 to enable mRNA export

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TREX exposes the RNA binding domain of Nxf1 to enable mRNA export

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Title: TREX exposes the RNA binding domain of Nxf1 to enable mRNA export
Author: Viphakone, Nicolas; Hautbergue, Guillaume M.; Walsh, Matthew; Chang, Chung-Te; Holland, Arthur; Folco, Eric G.; Reed, Robin Elizabeth; Wilson, Stuart A.

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Citation: Viphakone, Nicolas, Guillaume M. Hautbergue, Matthew Walsh, Chung-Te Chang, Arthur Holland, Eric G. Folco, Robin Reed, and Stuart A. Wilson. 2013. TREX exposes the RNA binding domain of Nxf1 to enable mRNA export. Nature communications 3: 1006.
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Abstract: The metazoan TREX complex is recruited to mRNA during nuclear RNA processing and functions in exporting mRNA to the cytoplasm. Nxf1 is an mRNA export receptor, which binds processed mRNA and transports it through the nuclear pore complex. At present, the relationship between TREX and Nxf1 is not understood. Here we show that Nxf1 uses an intramolecular interaction to inhibit its own RNA binding activity. When the TREX subunits Aly and Thoc5 make contact with Nxf1, Nxf1 is driven into an open conformation, exposing its RNA binding domain, allowing RNA binding. Moreover, the combined knockdown of Aly and Thoc5 drastically reduces the amount of Nxf1 bound to mRNA in vivo and also causes a severe mRNA export block. Together, our data indicate that TREX provides a license for mRNA export by driving Nxf1 into a conformation capable of binding mRNA.
Published Version: doi:10.1038/ncomms2005
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654228/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11181025
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