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dc.contributor.authorGuo, Shu-Zhen
dc.contributor.authorZhou, Yiming
dc.contributor.authorXing, Changhong
dc.contributor.authorLok, Josephine M.
dc.contributor.authorSom, Angel T.
dc.contributor.authorNing, MingMing
dc.contributor.authorJi, Xunming
dc.contributor.authorLo, Eng H.
dc.date.accessioned2013-10-18T12:57:06Z
dc.date.issued2012
dc.identifier.citationGuo, Shuzhen, Yiming Zhou, Changhong Xing, Josephine Lok, Angel T. Som, MingMing Ning, Xunming Ji, and Eng H. Lo. 2012. The vasculome of the mouse brain. PLoS ONE 7(12): e52665.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11181098
dc.description.abstractThe blood vessel is no longer viewed as passive plumbing for the brain. Increasingly, experimental and clinical findings suggest that cerebral endothelium may possess endocrine and paracrine properties – actively releasing signals into and receiving signals from the neuronal parenchyma. Hence, metabolically perturbed microvessels may contribute to central nervous system (CNS) injury and disease. Furthermore, cerebral endothelium can serve as sensors and integrators of CNS dysfunction, releasing measurable biomarkers into the circulating bloodstream. Here, we define and analyze the concept of a brain vasculome, i.e. a database of gene expression patterns in cerebral endothelium that can be linked to other databases and systems of CNS mediators and markers. Endothelial cells were purified from mouse brain, heart and kidney glomeruli. Total RNA were extracted and profiled on Affymetrix mouse 430 2.0 micro-arrays. Gene expression analysis confirmed that these brain, heart and glomerular preparations were not contaminated by brain cells (astrocytes, oligodendrocytes, or neurons), cardiomyocytes or kidney tubular cells respectively. Comparison of the vasculome between brain, heart and kidney glomeruli showed that endothelial gene expression patterns were highly organ-dependent. Analysis of the brain vasculome demonstrated that many functionally active networks were present, including cell adhesion, transporter activity, plasma membrane, leukocyte transmigration, Wnt signaling pathways and angiogenesis. Analysis of representative genome-wide-association-studies showed that genes linked with Alzheimer’s disease, Parkinson’s disease and stroke were detected in the brain vasculome. Finally, comparison of our mouse brain vasculome with representative plasma protein databases demonstrated significant overlap, suggesting that the vasculome may be an important source of circulating signals in blood. Perturbations in cerebral endothelial function may profoundly affect CNS homeostasis. Mapping and dissecting the vasculome of the brain in health and disease may provide a novel database for investigating disease mechanisms, assessing therapeutic targets and exploring new biomarkers for the CNS.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0052665en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527566/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectGenomicsen_US
dc.subjectGenome Expression Analysisen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectGene Expressionen_US
dc.subjectNeuroscienceen_US
dc.subjectDevelopmental Neuroscienceen_US
dc.subjectNeural Stem Cellsen_US
dc.subjectNeurogenesisen_US
dc.subjectCellular Neuroscienceen_US
dc.subjectMolecular Neuroscienceen_US
dc.subjectNeurobiology of Disease and Regenerationen_US
dc.subjectMedicineen_US
dc.subjectCardiovascularen_US
dc.subjectVascular Biologyen_US
dc.subjectNeurologyen_US
dc.subjectCerebrovascular Diseasesen_US
dc.subjectNeurodegenerative Diseasesen_US
dc.titleThe Vasculome of the Mouse Brainen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorLo, Eng H.
dc.date.available2013-10-18T12:57:06Z
dc.identifier.doi10.1371/journal.pone.0052665*
dash.contributor.affiliatedZhou, Yiming
dash.contributor.affiliatedGuo, Shu-Zhen
dash.contributor.affiliatedXing, Changhong
dash.contributor.affiliatedLok, Josephine M.
dash.contributor.affiliatedNing, MingMing
dash.contributor.affiliatedLo, Eng


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