Toward Multiplex Genome Engineering in Mammalian Cells
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CitationRios Villanueva, Xavier. 2013. Toward Multiplex Genome Engineering in Mammalian Cells. Doctoral dissertation, Harvard University.
AbstractGiven the explosion in human genetic data, new high-throughput genetic methods are necessary for studying variants and elucidating their role in human disease. In Chapter I, I will expand on this concept and describe current methods for genetically modifying human cells. In E. coli, Multiplex Automatable Genome Engineering (MAGE) is a powerful tool that enables the targeting of multiple genomic loci simultaneously with synthetic oligos that are recombined at high frequencies in an optimized strain. MAGE as a method has two components: organism-specific optimization of oligo recombination parameters and a protein capable of increasing recombination frequencies.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11181203
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