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dc.contributor.authorCârţână, Tatiana
dc.contributor.authorSăftoiu, Adrian
dc.contributor.authorGruionu, Lucian Gheorghe
dc.contributor.authorGheonea, Dan Ionuţ
dc.contributor.authorPirici, Daniel
dc.contributor.authorGeorgescu, Claudia Valentina
dc.contributor.authorCiocâlteu, Adriana
dc.contributor.authorGruionu, Gabriel
dc.date.accessioned2013-10-18T14:50:18Z
dc.date.issued2012
dc.identifier.citationCârţână, Tatiana, Adrian Săftoiu, Lucian Gheorghe Gruionu, Dan Ionuţ Gheonea, Daniel Pirici, Claudia Valentina Georgescu, Adriana Ciocâlteu, and Gabriel Gruionu. 2012. Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS ONE 7(12): e52815.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11181208
dc.description.abstractIntroduction: Numerous anti-angiogenic agents are currently developed to limit tumor growth and metastasis. While these drugs offer hope for cancer patients, their transient effect on tumor vasculature is difficult to assess in clinical settings. Confocal laser endomicroscopy (CLE) is a novel endoscopic imaging technology that enables histological examination of the gastrointestinal mucosa. The aim of the present study was to evaluate the feasibility of using CLE to image the vascular network in fresh biopsies of human colorectal tissue. For this purpose we have imaged normal and malignant biopsy tissue samples and compared the vascular network parameters obtained with CLE with established histopathology techniques. Materials and Methods: Fresh non-fixed biopsy samples of both normal and malignant colorectal mucosa were stained with fluorescently labeled anti-CD31 antibodies and imaged by CLE using a dedicated endomicroscopy system. Corresponding biopsy samples underwent immunohistochemical staining for CD31, assessing the microvessel density (MVD) and vascular areas for comparison with CLE data, which were measured offline using specific software. Results: The vessels were imaged by CLE in both normal and tumor samples. The average diameter of normal vessels was 8.5±0.9 µm whereas in tumor samples it was 13.5±0.7 µm (p = 0.0049). Vascular density was 188.7±24.9 vessels/mm\(^2\) in the normal tissue vs. 242.4±16.1 vessels/mm\(^2\) in the colorectal cancer samples (p = 0.1201). In the immunohistochemistry samples, the MVD was 211.2±42.9/mm\(^2\) and 351.3±39.6/mm\(^2\) for normal and malignant mucosa, respectively. The vascular area was 2.9±0.5% of total tissue area for the normal mucosa and 8.5±2.1% for primary colorectal cancer tissue. Conclusion: Selective imaging of blood vessels with CLE is feasible in normal and tumor colorectal tissue by using fluorescently labeled antibodies targeted against an endothelial marker. The method could be translated into the clinical setting for monitoring of anti-angiogenic therapy.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0052815en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532115/pdf/en_US
dash.licenseLAA
dc.subjectMedicineen_US
dc.subjectDiagnostic Medicineen_US
dc.subjectPathologyen_US
dc.subjectGeneral Pathologyen_US
dc.subjectMolecular Pathologyen_US
dc.subjectGastroenterology and Hepatologyen_US
dc.subjectColonen_US
dc.subjectEndoscopyen_US
dc.subjectGastrointestinal Cancersen_US
dc.subjectOncologyen_US
dc.subjectCancers and Neoplasmsen_US
dc.subjectGastrointestinal Tumorsen_US
dc.subjectColon Adenocarcinomaen_US
dc.subjectBasic Cancer Researchen_US
dc.titleConfocal Laser Endomicroscopy for the Morphometric Evaluation of Microvessels in Human Colorectal Cancer Using Targeted Anti-CD31 Antibodiesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorGruionu, Gabriel
dc.date.available2013-10-18T14:50:18Z
dc.identifier.doi10.1371/journal.pone.0052815*
dash.authorsorderedfalse
dash.contributor.affiliatedGruionu, Gabriel


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