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dc.contributor.authorEroshenko, Nikolai Alexandrovich
dc.contributor.authorRamachandran, Rukmani
dc.contributor.authorYadavalli, Vamsi K
dc.contributor.authorRao, Rajesh Raghavendran
dc.date.accessioned2013-10-22T18:34:04Z
dc.date.issued2013
dc.identifier.citationEroshenko, Nikolai, Rukmani Ramachandran, Vamsi K Yadavalli, and Raj R Rao. 2013. Effect of substrate stiffness on early human embryonic stem cell differentiation. Journal of Biological Engineering 7: 7.en_US
dc.identifier.issn1754-1611en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11193283
dc.description.abstractBackground: The pluripotency and self renewing properties of human embryonic stem cells (hESC) make them a valuable tool in the fields of developmental biology, pharmacology and regenerative medicine. Therefore, there exists immense interest in devising strategies for hESC propagation and differentiation. Methods involving simulation of the native stem cell microenvironment, both chemical and physical, have received a lot of attention in recent years. Equally important is evidence that cells can also sense the mechanical properties of their microenvironment. In this study, we test the hypothesis that hESCs accept mechanical cues for differentiation from the substrate by culturing them on flexible polydimethylsiloxane (PDMS) of varying stiffness. Results: PDMS substrates were prepared using available commercial formulations and characterized for stiffness, surface properties and efficiency of cell attachment and proliferation. Across different substrate stiffness, cell numbers, cell attachment and cell surface area were found to be similar. Expression of pluripotency markers decreased with increased time in culture across all PDMS substrates of varying stiffness. Analysis of gene expression of differentiation markers indicates that the differentiation process becomes less stochastic with longer culture times. Conclusions: We evaluated the utility of PDMS substrates for stem cell propagation and substrate mediated differentiation. The stiffness affected gene expression of pluripotent and differentiation markers with results indicating that these substrate systems could potentially be used to direct hESC fate towards early mesodermal lineages. This study suggests that coupled with soluble factors, PDMS substrates could potentially be useful in generating defined populations of differentiated cells.en_US
dc.description.sponsorshipEngineering and Applied Sciencesen_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1754-1611-7-7en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621683/pdf/en_US
dash.licenseLAA
dc.subjectStem cellsen_US
dc.subjectBiomaterialsen_US
dc.subjectExtracellular matrixen_US
dc.subjectDifferentiationen_US
dc.subjectStiffnessen_US
dc.titleEffect of substrate stiffness on early human embryonic stem cell differentiationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Biological Engineeringen_US
dash.depositing.authorRao, Rajesh Raghavendran
dc.date.available2013-10-22T18:34:04Z
dc.identifier.doi10.1186/1754-1611-7-7*
workflow.legacycommentsFLAG8 should be HMSen_US
dash.contributor.affiliatedRao, Rajesh Raghavendran
dash.contributor.affiliatedEroshenko, Nikolai Alexandrovich


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