RecA Homology Search is Promoted by Mechanical Stress Along the Scanned Duplex DNA
Bishop, Douglas K.
MetadataShow full item record
CitationDanilowicz, Claudia, Efraim Feinstein, Alyson Conover, Vincent W. Coljee, Julea Vlassakis, Yuen-Ling Chan, Douglas K. Bishop, and Mara Prentiss. 2011. RecA homology search is promoted by mechanical stress along the scanned duplex DNA. Nucleic Acids Research 40, no. 4: 1717-1727.
AbstractA RecA–single-stranded DNA (RecA–ssDNA) filament searches a genome for sequence homology by rapidly binding and unbinding double-stranded DNA (dsDNA) until homology is found. We demonstrate that pulling on the opposite termini (3′ and 5′) of one of the two DNA strands in a dsDNA molecule stabilizes the normally unstable binding of that dsDNA to non-homologous RecA–ssDNA filaments, whereas pulling on the two 3′, the two 5′, or all four termini does not. We propose that the ‘outgoing’ strand in the dsDNA is extended by strong DNA–protein contacts, whereas the ‘complementary’ strand is extended by the tension on the base pairs that connect the ‘complementary’ strand to the ‘outgoing’ strand. The stress resulting from different levels of tension on its constitutive strands causes rapid dsDNA unbinding unless sufficient homology is present.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11210457
- FAS Scholarly Articles