RecA Homology Search is Promoted by Mechanical Stress Along the Scanned Duplex DNA

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RecA Homology Search is Promoted by Mechanical Stress Along the Scanned Duplex DNA

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Title: RecA Homology Search is Promoted by Mechanical Stress Along the Scanned Duplex DNA
Author: Conover, Alyson; Vlassakis, Julea; Chan, Yuen-Ling; Bishop, Douglas K.; Danilowicz, Claudia; Feinstein, Efraim David; Coljee, Vincent William; Prentiss, Mara

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Citation: Danilowicz, Claudia, Efraim Feinstein, Alyson Conover, Vincent W. Coljee, Julea Vlassakis, Yuen-Ling Chan, Douglas K. Bishop, and Mara Prentiss. 2011. RecA homology search is promoted by mechanical stress along the scanned duplex DNA. Nucleic Acids Research 40, no. 4: 1717-1727.
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Abstract: A RecA–single-stranded DNA (RecA–ssDNA) filament searches a genome for sequence homology by rapidly binding and unbinding double-stranded DNA (dsDNA) until homology is found. We demonstrate that pulling on the opposite termini (3′ and 5′) of one of the two DNA strands in a dsDNA molecule stabilizes the normally unstable binding of that dsDNA to non-homologous RecA–ssDNA filaments, whereas pulling on the two 3′, the two 5′, or all four termini does not. We propose that the ‘outgoing’ strand in the dsDNA is extended by strong DNA–protein contacts, whereas the ‘complementary’ strand is extended by the tension on the base pairs that connect the ‘complementary’ strand to the ‘outgoing’ strand. The stress resulting from different levels of tension on its constitutive strands causes rapid dsDNA unbinding unless sufficient homology is present.
Published Version: doi://10.1093/nar/gkr855
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287184/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11210457
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