# Sequencing by Hybridization of Long Targets

 Title: Sequencing by Hybridization of Long Targets Author: Qin, Yu; Schneider, Tobias M; Brenner, Michael P. Note: Order does not necessarily reflect citation order of authors. Citation: Qin, Yu, Tobias M. Schneider, and Michael P. Brenner. 2012. Sequencing by hybridization of long targets. PLoS ONE 7(5): e35819. Full Text & Related Files: 3344849.pdf (690.5Kb; PDF) Abstract: Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined $$l$$-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to $$n=O(2^{l})$$ due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error. Published Version: doi:10.1371/journal.pone.0035819 Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344849/pdf/ Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11210605 Downloads of this work: