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dc.contributor.authorGrimes, Catherine Leimkuhler
dc.contributor.authorAriyananda, Lushanti De Zoysa
dc.contributor.authorMelnyk, James E.
dc.contributor.authorO'Shea, Erin K
dc.date.accessioned2013-10-24T19:25:59Z
dc.date.issued2012
dc.identifier.citationGrimes, Catherine Leimkuhler, Lushanti De Zoysa Ariyananda, James E. Melnyk, and Erin K. O’Shea. 2012. The innate immune protein nod2 binds directly to mdp, a bacterial cell wall fragment. Journal of the American Chemical Society 134(33): 13535-13537.en_US
dc.identifier.issn0002-7863en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11210616
dc.description.abstractMammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn’s disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptide (MDP), an immunostimulatory fragment of bacterial cell wall, but it is not known whether MDP binds directly to Nod2. We report the expression and purification of human Nod2 from insect cells. Using novel MDP self-assembled monolayers (SAMs), we provide the first biochemical evidence for a direct, high-affinity interaction between Nod2 and MDP.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofdoi:10.1021/ja303883cen_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424850/pdf/en_US
dash.licenseLAA
dc.titleThe Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragmenten_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalJournal of the American Chemical Societyen_US
dash.depositing.authorO'Shea, Erin K
dash.waiver2012-08-24
dc.date.available2013-10-24T19:25:59Z
dc.identifier.doi10.1021/ja303883c*
workflow.legacycommentsArticle published under the open ACS choice license http://pubs.acs.org/page/policy/authorchoice/index.htmlen_US
dash.authorsorderedfalse
dash.contributor.affiliatedO'Shea, Erin


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