Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours

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Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours

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Title: Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours
Author: Glenn, David R.; Zhang, Huidan; Kasthuri, Narayanan; Schalek, Richard L.; Lo, P. K.; Trifonov, Alexei; Park, Hongkun; Lichtman, Jeff; Walsworth, Ronald L.

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Citation: Glenn, D. R., H. Zhang, N. Kasthuri, R. Schalek, P. K. Lo, A. S. Trifonov, H. Park, J. W. Lichtman, and R. L. Walsworth. 2012. Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours. Scientific Reports 2:865.
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Abstract: Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images, and rapid optical signal degradation under electron beam irradiation. Here, we introduce a new approach to solve these problems: imaging of stable optical cathodoluminescence emitted in a scanning electron microscope by nanoparticles with controllable surface chemistry. We demonstrate well-correlated cathodoluminescence and secondary electron images using three species of semiconductor nanoparticles that contain defects providing stable, spectrally-distinguishable cathodoluminescence. We also demonstrate reliable surface functionalization of the particles. The results pave the way for the use of such nanoparticles for targeted labeling of surfaces to provide nanoscale mapping of molecular composition, indicated by cathodoluminescence colour, simultaneously acquired with structural electron images in a single instrument.
Published Version: doi:10.1038/srep00865
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498735/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11210617
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