Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
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Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
| Title: | Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment |
| Author: |
McGregor, Lynn Marie; Gorin, David Joel; Dumelin, Christoph; Liu, David Ruchien
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | McGregor, Lynn M., David J. Gorin, Christoph E. Dumelin, and David R. Liu. 2010. Interaction-Dependent PCR: Identification of Ligandâ Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment. Journal of the American Chemical Society 132(44): 15522-15524. |
| Full Text & Related Files: |
2974369.pdf (829.4Kb; PDF) 
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| Abstract: | Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences. |
| Published Version: | doi:10.1021/ja107677q |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974369/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11211555 |
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