Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
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CitationMcGregor, Lynn M., David J. Gorin, Christoph E. Dumelin, and David R. Liu. 2010. Interaction-Dependent PCR: Identification of Ligandâ Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment. Journal of the American Chemical Society 132(44): 15522-15524.
AbstractInteraction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.
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