Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

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Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

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Title: Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
Author: McGregor, Lynn Marie; Gorin, David Joel; Dumelin, Christoph; Liu, David Ruchien

Note: Order does not necessarily reflect citation order of authors.

Citation: McGregor, Lynn M., David J. Gorin, Christoph E. Dumelin, and David R. Liu. 2010. Interaction-Dependent PCR: Identification of Ligandâ Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment. Journal of the American Chemical Society 132(44): 15522-15524.
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Abstract: Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.
Published Version: doi:10.1021/ja107677q
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974369/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11211555
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