Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment

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Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment

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Title: Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment
Author: Gopalakrishnan, Jayachandran; Chim, Yiu-Cheung Frederick; Ha, Andrew; Basiri, Marcus L.; Lerit, Dorothy A.; Rusan, Nasser M.; Avidor-Reiss, Tomer

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Citation: Gopalakrishnan, Jayachandran, Yiu-Cheung Frederick Chim, Andrew Ha, Marcus L. Basiri, Dorothy A. Lerit, Nasser M. Rusan, and Tomer Avidor-Reiss. 2012. Tubulin nucleotide status controls sas-4-dependent pericentriolar material recruitment. Nature cell biology 14(8): 865-873.
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Abstract: Regulated centrosome biogenesis is required for accurate cell division and for maintaining genome integrity1. Centrosomes consist of a centriole pair surrounded by a protein network known as pericentriolar material (PCM)1. PCM assembly is a tightly regulated, critical step that determines a centrosome’s size and capability2–4. Here, we report a role for tubulin in regulating PCM recruitment via the conserved centrosomal protein Sas-4. Tubulin directly binds to Sas-4; together they are components of cytoplasmic complexes of centrosomal proteins5,6. A Sas-4 mutant, which cannot bind tubulin, enhances centrosomal protein complex formation and has abnormally large centrosomes with excessive activity. These suggest that tubulin negatively regulates PCM recruitment. Whereas tubulin-GTP prevents Sas-4 from forming protein complexes, tubulin-GDP promotes it. Thus, tubulin’s regulation of PCM recruitment depends on its GTP/GDP-bound state. These results identify a role for tubulin in regulating PCM recruitment independent of its well-known role as a building block of microtubules7. Based on its guanine bound state, tubulin can act as a molecular switch in PCM recruitment.
Published Version: doi:10.1038/ncb2527
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411905/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11235648
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