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dc.contributor.authorMilner, Danny Arnold
dc.contributor.authorVareta, Jimmy
dc.contributor.authorValim, Clarissa
dc.contributor.authorMontgomery, Jacqui
dc.contributor.authorDaniels, Rachel Fath
dc.contributor.authorCooke, Sarah Volkman
dc.contributor.authorNeafsey, Daniel Edward
dc.contributor.authorPark, Daniel John
dc.contributor.authorSchaffner, Stephen
dc.contributor.authorMahesh, Nira C
dc.contributor.authorBarnes, Kayla G
dc.contributor.authorRosen, David M
dc.contributor.authorLukens, Amanda Kathleen
dc.contributor.authorVan-Tyne, Daria
dc.contributor.authorWiegand, Roger C.
dc.contributor.authorSabeti, Pardis Christine
dc.contributor.authorSeydel, Karl B
dc.contributor.authorGlover, Simon J
dc.contributor.authorKamiza, Steve
dc.contributor.authorMolyneux, Malcolm E
dc.contributor.authorTaylor, Terrie E
dc.contributor.authorWirth, Dyann Fergus
dc.date.accessioned2013-11-01T17:10:09Z
dc.date.issued2012
dc.identifier.citationMilner, Danny A, Jimmy Vareta, Clarissa Valim, Jacqui Montgomery, Rachel F Daniels, Sarah K Volkman, Daniel E Neafsey, et al. 2012. Human cerebral malaria and Plasmodium falciparum genotypes in Malawi. Malaria Journal 11:35.en_US
dc.identifier.issn1475-2875en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11248785
dc.description.abstractBackground: Cerebral malaria, a severe form of Plasmodium falciparum infection, is an important cause of mortality in sub-Saharan African children. A Taqman 24 Single Nucleotide Polymorphisms (SNP) molecular barcode assay was developed for use in laboratory parasites which estimates genotype number and identifies the predominant genotype. Methods The 24 SNP assay was used to determine predominant genotypes in blood and tissues from autopsy and clinical patients with cerebral malaria. Results: Single genotypes were shared between the peripheral blood, the brain, and other tissues of cerebral malaria patients, while malaria-infected patients who died of non-malarial causes had mixed genetic signatures in tissues examined. Children with retinopathy-positive cerebral malaria had significantly less complex infections than those without retinopathy (OR = 3.7, 95% CI [1.51-9.10]).The complexity of infections significantly decreased over the malaria season in retinopathy-positive patients compared to retinopathy-negative patients. Conclusions: Cerebral malaria patients harbour a single or small set of predominant parasites; patients with incidental parasitaemia sustain infections involving diverse genotypes. Limited diversity in the peripheral blood of cerebral malaria patients and correlation with tissues supports peripheral blood samples as appropriate for genome-wide association studies of parasite determinants of pathogenicity.en_US
dc.description.sponsorshipOrganismic and Evolutionary Biologyen_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1475-2875-11-35en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3295736/pdf/en_US
dash.licenseLAA
dc.subjectPlasmodium falciparumen_US
dc.subjectcerebral malariaen_US
dc.subjectgenotypingen_US
dc.subjectmolecular barcodeen_US
dc.subjecthistopathologyen_US
dc.subjectautopsyen_US
dc.titleHuman Cerebral Malaria and Plasmodium falciparum Genotypes in Malawien_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalMalaria Journalen_US
dash.depositing.authorSabeti, Pardis Christine
dc.date.available2013-11-01T17:10:09Z
dc.identifier.doi10.1186/1475-2875-11-35*
workflow.legacycommentsFLAG8 HSPHen_US
dash.authorsorderedfalse
dash.contributor.affiliatedWiegand, Roger
dash.contributor.affiliatedVolkman, Sarah
dash.contributor.affiliatedLukens, Amanda
dash.contributor.affiliatedDaniels, Rachel
dash.contributor.affiliatedValim, Clarissa
dash.contributor.affiliatedNeafsey, Daniel
dash.contributor.affiliatedWirth, Dyann
dash.contributor.affiliatedMilner, Danny
dash.contributor.affiliatedPark, Daniel John
dash.contributor.affiliatedSchaffner, Stephen
dash.contributor.affiliatedSabeti, Pardis


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