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dc.contributor.authorZhao, Mingwei
dc.contributor.authorZhang, Jiangwen
dc.contributor.authorPhatnani, Hemali
dc.contributor.authorScheu, Stefanie
dc.contributor.authorManiatis, Thomas P.
dc.date.accessioned2013-11-01T17:32:17Z
dc.date.issued2012
dc.identifier.citationZhao, Mingwei, Jiangwen Zhang, Hemali Phatnani, Stefanie Scheu, and Tom Maniatis. 2012. Stochastic expression of the interferon-β gene. PLoS Biology 10(1): e1001249.en_US
dc.identifier.issn1544-9173en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11248786
dc.description.abstractVirus infection of mammalian cells induces the production of high levels of type I interferons (IFNα and β), cytokines that orchestrate antiviral innate and adaptive immunity. Previous studies have shown that only a fraction of the infected cells produce IFN. However, the mechanisms responsible for this stochastic expression are poorly understood. Here we report an in depth analysis of IFN-expressing and non-expressing mouse cells infected with Sendai virus. Mouse embryonic fibroblasts in which an internal ribosome entry site/yellow fluorescent protein gene was inserted downstream from the endogenous IFNβ gene were used to distinguish between the two cell types, and they were isolated from each other using fluorescence-activated cell sorting methods. Analysis of the separated cells revealed that stochastic IFNβ expression is a consequence of cell-to-cell variability in the levels and/or activities of limiting components at every level of the virus induction process, ranging from viral replication and expression, to the sensing of viral RNA by host factors, to activation of the signaling pathway, to the levels of activated transcription factors. We propose that this highly complex stochastic IFNβ gene expression evolved to optimize both the level and distribution of type I IFNs in response to virus infection.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pbio.1001249en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265471/pdf/en_US
dash.licenseLAA
dc.subjectbiologyen_US
dc.subjectgeneticsen_US
dc.subjectmolecular geneticsen_US
dc.subjectimmunologyen_US
dc.subjectimmune systemen_US
dc.subjectimmunityen_US
dc.subjectmicrobiologyen_US
dc.subjectvirologyen_US
dc.subjectmolecular cell biologyen_US
dc.subjectsignal transductionen_US
dc.subjectsignaling in cellular processesen_US
dc.subjectsystems biologyen_US
dc.titleStochastic Expression of the Interferon-β Geneen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Biologyen_US
dash.depositing.authorManiatis, Thomas P.
dc.date.available2013-11-01T17:32:17Z
dc.identifier.doi10.1371/journal.pbio.1001249*
dash.contributor.affiliatedZhang, Jiangwen
dash.contributor.affiliatedManiatis, Thomas


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