Alterations of Serum Levels of BDNF-Related miRNAs in Patients with Depression

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Alterations of Serum Levels of BDNF-Related miRNAs in Patients with Depression

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Title: Alterations of Serum Levels of BDNF-Related miRNAs in Patients with Depression
Author: Li, You-Jie; Xu, Mei; Gao, Zong-Hua; Wang, Ya-Qi; Yue, Zhen; Zhang, Yan-Xia; Li, Xin-Xin; Zhang, Can; Xie, Shu-Yang; Wang, Ping-Yu

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Citation: Li, You-Jie, Mei Xu, Zong-Hua Gao, Ya-Qi Wang, Zhen Yue, Yan-Xia Zhang, Xin-Xin Li, Can Zhang, Shu-Yang Xie, and Ping-Yu Wang. 2013. Alterations of serum levels of bdnf-related mirnas in patients with depression. PLoS ONE 8(5): e63648.
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Abstract: Depression is a serious and potentially life-threatening mental disorder with unknown etiology. Emerging evidence shows that brain-derived neurotrophic factor (BDNF) and microRNAs (miRNAs) play critical roles in the etiology of depression. Here this study was aimed to identify and characterize the roles of BDNF and its putative regulatory miRNAs in depression. First, we identified that miR-182 may be a putative miRNA that regulates BDNF levels by bioinformatic studies, and characterized the effects of miR-182 on the BDNF levels using cell-based studies, side by side with miR-132 (a known miRNA that regulates BDNF expression). We showed that treatment of miR-132 and miR-182 respectively decreased the BDNF protein levels in a human neuronal cell model, supporting the regulatory roles of miR-132 and miR-182 on the BDNF expression. Furthermore, we explored the roles of miR-132 and miR-182 on the BDNF levels in depression using human subjects by assessing their serum levels. Compared with the healthy controls, patients with depression showed lower serum BDNF levels (via the enzyme-linked immunosorbent assays) and higher serum miR-132 and miR-182 levels (via the real-time PCR). Finally, the Pearson’s (or Spearman’s) correlation coefficient was calculated to study whether there was a relationship among the Self-Rating Depression Scale score, the serum BDNF levels, and serum BDNF-related miRNA levels. Our results revealed that there was a significant negative correlation between the SDS scores and the serum BDNF levels, and a positive correlation between the SDS scores and miR-132 levels. In addition, we found a reverse relationship between the serum BDNF levels and the miR-132/miR-182 levels in depression. Collectively, we provided evidence supporting that miR-182 is a putative BDNF-regulatory miRNA, and suggested that the serum BDNF and its related miRNAs may be utilized as important biomarkers in the diagnosis or as therapeutic targets of depression.
Published Version: doi:10.1371/journal.pone.0063648
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660391/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11357497
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