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dc.contributor.authorDoerner, Julia
dc.contributor.authorFebvay, Sebastien
dc.contributor.authorClapham, David Eldon
dc.date.accessioned2013-12-10T15:34:39Z
dc.date.issued2013
dc.identifier.citationDoerner, Julia F., Sebastien Febvay, and David E. Clapham. 2013. Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel mscl. Nature communications 3: 990.en_US
dc.identifier.issn2041-1723en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11363090
dc.description.abstractBacterial mechanosensitive channels are some of the largest pores in nature. In particular, MscL, with a pore diameter > 25 Å, allows passage of large organic ions and small proteins. Functional MscL reconstitution into lipids has been proposed for applications in vesicular-based drug release. Here we show that these channels can be functionally expressed in mammalian cells to afford rapid controlled uptake of membrane impermeable molecules. We first demonstrate that MscL gating in response to increased membrane tension is preserved in mammalian cell membranes. Molecular delivery is controlled by adopting an established method of MscL charge-induced activation. We then determine pore size limitations using fluorescently labeled model cargoes. Finally, we activate MscL to introduce the cell-impermeable bi-cyclic peptide phalloidin, a specific marker for actin filaments, into cells. We propose that MscL will be a useful tool for gated and controlled delivery of bioactive molecules into cells.en_US
dc.language.isoen_USen_US
dc.relation.isversionofdoi:10.1038/ncomms1999en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651673/pdf/en_US
dash.licenseLAA
dc.titleControlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscLen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNature communicationsen_US
dash.depositing.authorDoerner, Julia
dc.date.available2013-12-10T15:34:39Z
dc.identifier.doi10.1038/ncomms1999*
dash.contributor.affiliatedDoerner, Julia F
dash.contributor.affiliatedFebvay, Sebastien
dash.contributor.affiliatedClapham, David


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