In Vivo Time-Gated Fluorescence Imaging With Biodegradable Luminescent Porous Silicon Nanoparticles

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In Vivo Time-Gated Fluorescence Imaging With Biodegradable Luminescent Porous Silicon Nanoparticles

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Title: In Vivo Time-Gated Fluorescence Imaging With Biodegradable Luminescent Porous Silicon Nanoparticles
Author: Gu, Luo; Hall, David J.; Qin, Zhengtao; Anglin, Emily; Joo, Jinmyoung; Mooney, David J.; Howell, Stephen B.; Sailor, Michael J.

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Citation: Gu, Luo, David J. Hall, Zhengtao Qin, Emily Anglin, Jinmyoung Joo, David J. Mooney, Stephen B. Howell, and Michael J. Sailor. 2013. In vivo time-gated fluorescence imaging with biodegradable luminescent porous silicon nanoparticles. Nature Communications 4: 2326.
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Abstract: Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5–13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (<10 ns) emission signals from organic chromophores or tissue autofluorescence. Here using a conventional animal imaging system not optimized for such long-lived excited states, we demonstrate improvement of signal to background contrast ratio by >50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.
Published Version: doi:10.1038/ncomms3326
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11591711
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