Jones, Siân, Wei-dong Chen, Giovanni Parmigiani, Frank Diehl, Niko Beerenwinkel, Tibor Antal, Arne Traulsen, et al. 2008. Comparative lesion sequencing provides insights into tumor evolution. Proceedings of the National Academy of Sciences 105(11): 4283-4288.
We show that the times separating the birth of benign, invasive, and metastatic tumor cells can be determined by analysis of the mutations they have in common. When combined with prior clinical observations, these analyses suggest the following general conclusions about colorectal tumorigenesis: (i) It takes ≈17 years for a large benign tumor to evolve into an advanced cancer but <2 years for cells within that cancer to acquire the ability to metastasize; (ii) it requires few, if any, selective events to transform a highly invasive cancer cell into one with the capacity to metastasize; (iii) the process of cell culture ex vivo does not introduce new clonal mutations into colorectal tumor cell populations; and (iv) the rates at which point mutations develop in advanced cancers are similar to those of normal cells. These results have important implications for understanding human tumor pathogenesis, particularly those associated with metastasis.
FLAG2. Published version cannot be posted, and the article was likely completed in Q4 of 2007 prior to the passage of the OAP. But, if we had a manuscript, we could post LAA. In QSDB. http://www.sherpa.ac.uk/romeo/issn/1091-6490/; manuscript received from assistant (Soma Roy)