MCU encodes the pore conducting mitochondrial calcium currents
Citation
Chaudhuri, Dipayan, Yasemin Sancak, Vamsi K Mootha, and David E Clapham. 2013. “MCU encodes the pore conducting mitochondrial calcium currents.” eLife 2 (1): e00704. doi:10.7554/eLife.00704. http://dx.doi.org/10.7554/eLife.00704.Abstract
Mitochondrial calcium (Ca2+) import is a well-described phenomenon regulating cell survival and ATP production. Of multiple pathways allowing such entry, the mitochondrial Ca2+ uniporter is a highly Ca2+-selective channel complex encoded by several recently-discovered genes. However, the identity of the pore-forming subunit remains to be established, since knockdown of all the candidate uniporter genes inhibit Ca2+ uptake in imaging assays, and reconstitution experiments have been equivocal. To definitively identify the channel, we use whole-mitoplast voltage-clamping, the technique that originally established the uniporter as a Ca2+ channel. We show that RNAi-mediated knockdown of the mitochondrial calcium uniporter (MCU) gene reduces mitochondrial Ca2+ current (IMiCa), whereas overexpression increases it. Additionally, a classic feature of IMiCa, its sensitivity to ruthenium red inhibition, can be abolished by a point mutation in the putative pore domain without altering current magnitude. These analyses establish that MCU encodes the pore-forming subunit of the uniporter channel. DOI: http://dx.doi.org/10.7554/eLife.00704.001Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3673318/pdf/Terms of Use
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