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dc.contributor.authorChaudhuri, Dipayanen_US
dc.contributor.authorSancak, Yaseminen_US
dc.contributor.authorMootha, Vamsi Ken_US
dc.contributor.authorClapham, David Een_US
dc.date.accessioned2014-02-13T19:00:53Z
dc.date.issued2013en_US
dc.identifier.citationChaudhuri, Dipayan, Yasemin Sancak, Vamsi K Mootha, and David E Clapham. 2013. “MCU encodes the pore conducting mitochondrial calcium currents.” eLife 2 (1): e00704. doi:10.7554/eLife.00704. http://dx.doi.org/10.7554/eLife.00704.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11708550
dc.description.abstractMitochondrial calcium (Ca2+) import is a well-described phenomenon regulating cell survival and ATP production. Of multiple pathways allowing such entry, the mitochondrial Ca2+ uniporter is a highly Ca2+-selective channel complex encoded by several recently-discovered genes. However, the identity of the pore-forming subunit remains to be established, since knockdown of all the candidate uniporter genes inhibit Ca2+ uptake in imaging assays, and reconstitution experiments have been equivocal. To definitively identify the channel, we use whole-mitoplast voltage-clamping, the technique that originally established the uniporter as a Ca2+ channel. We show that RNAi-mediated knockdown of the mitochondrial calcium uniporter (MCU) gene reduces mitochondrial Ca2+ current (IMiCa), whereas overexpression increases it. Additionally, a classic feature of IMiCa, its sensitivity to ruthenium red inhibition, can be abolished by a point mutation in the putative pore domain without altering current magnitude. These analyses establish that MCU encodes the pore-forming subunit of the uniporter channel. DOI: http://dx.doi.org/10.7554/eLife.00704.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.00704en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3673318/pdf/en
dash.licenseLAAen_US
dc.subjectmitoplasten
dc.subjectMICU1en
dc.subjectruthenium reden
dc.subjectcalcium channelen
dc.subjectelectrophysiologyen
dc.subjectMCUR1en
dc.subjectHumanen
dc.titleMCU encodes the pore conducting mitochondrial calcium currentsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorChaudhuri, Dipayanen_US
dc.date.available2014-02-13T19:00:53Z
dc.identifier.doi10.7554/eLife.00704*
dash.contributor.affiliatedChaudhuri, Dipayan
dash.contributor.affiliatedClapham, David
dash.contributor.affiliatedSancak, Yasemin
dash.contributor.affiliatedMootha, Vamsi


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