Evidence against a germ plasm in the milkweed bug Oncopeltus fasciatus, a hemimetabolous insect

DSpace/Manakin Repository

Evidence against a germ plasm in the milkweed bug Oncopeltus fasciatus, a hemimetabolous insect

Citable link to this page

 

 
Title: Evidence against a germ plasm in the milkweed bug Oncopeltus fasciatus, a hemimetabolous insect
Author: Ewen-Campen, Ben; Jones, Tamsin E. M.; Extavour, Cassandra G.

Note: Order does not necessarily reflect citation order of authors.

Citation: Ewen-Campen, Ben, Tamsin E. M. Jones, and Cassandra G. Extavour. 2013. “Evidence against a germ plasm in the milkweed bug Oncopeltus fasciatus, a hemimetabolous insect.” Biology Open 2 (6): 556-568. doi:10.1242/bio.20134390. http://dx.doi.org/10.1242/bio.20134390.
Full Text & Related Files:
Abstract: Summary Primordial germ cell (PGC) formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic nuclei that inherit this “germ plasm” acquire PGC fate. In contrast, historical studies of basally branching insects (Hemimetabola) suggest that a maternal requirement for germ line genes in PGC specification may be a derived character confined principally to Holometabola. However, there have been remarkably few investigations of germ line gene expression and function in hemimetabolous insects. Here we characterise PGC formation in the milkweed bug Oncopeltus fasciatus, a member of the sister group to Holometabola, thus providing an important evolutionary comparison to members of this clade. We examine the transcript distribution of orthologues of 19 Drosophila germ cell and/or germ plasm marker genes, and show that none of them localise asymmetrically within Oncopeltus oocytes or early embryos. Using multiple molecular and cytological criteria, we provide evidence that PGCs form after cellularisation at the site of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent with the hypothesis that germ plasm is a derived strategy of germ cell specification in insects.
Published Version: doi:10.1242/bio.20134390
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683158/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11708599
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters