Bypass of a protein roadblock by a replicative DNA helicase

DSpace/Manakin Repository

Bypass of a protein roadblock by a replicative DNA helicase

Citable link to this page

 

 
Title: Bypass of a protein roadblock by a replicative DNA helicase
Author: Yardimci, Hasan; Wang, Xindan; Loveland, Anna B.; Tappin, Inger; Rudner, David Z.; Hurwitz, Jerard; van Oijen, Antoine M.; Walter, Johannes C.

Note: Order does not necessarily reflect citation order of authors.

Citation: Yardimci, Hasan, Xindan Wang, Anna B. Loveland, Inger Tappin, David Z. Rudner, Jerard Hurwitz, Antoine M. van Oijen, and Johannes C. Walter. 2012. “Bypass of a protein roadblock by a replicative DNA helicase.” Nature 492 (7428): 205-209. doi:10.1038/nature11730. http://dx.doi.org/10.1038/nature11730.
Full Text & Related Files:
Abstract: Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (“steric exclusion”). In contrast, large T antigen (T-ag), the replicative DNA helicase of the Simian Virus 40 (SV40), is reported to function as a pair of stacked hexamers that pumps double-stranded DNA through its central channel while laterally extruding single-stranded DNA. Here, we use single-molecule and ensemble assays to show that T-ag assembled on the SV40 origin unwinds DNA efficiently as a single hexamer that translocates on single-stranded DNA in the 3′ to 5′ direction. Unexpectedly, T-ag unwinds DNA past a DNA-protein crosslink on the translocation strand, suggesting that the T-ag ring can open to bypass bulky adducts. Together, our data underscore the profound conservation among replicative helicase mechanisms while revealing a new level of plasticity in their interactions with DNA damage.
Published Version: doi:10.1038/nature11730
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521859/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11708610
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters