Localized Epigenetic Changes Induced by DH Recombination Restricts Recombinase to DJH Junctions

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Localized Epigenetic Changes Induced by DH Recombination Restricts Recombinase to DJH Junctions

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Title: Localized Epigenetic Changes Induced by DH Recombination Restricts Recombinase to DJH Junctions
Author: Subrahmanyam, Ramesh; Du, Hansen; Ivanova, Irina; Chakraborty, Tirtha; Ji, Yanhong; Zhang, Yu; Alt, Frederick W.; Schatz, David G.; Sen, Ranjan

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Citation: Subrahmanyam, Ramesh, Hansen Du, Irina Ivanova, Tirtha Chakraborty, Yanhong Ji, Yu Zhang, Frederick W. Alt, David G. Schatz, and Ranjan Sen. 2013. “Localized Epigenetic Changes Induced by DH Recombination Restricts Recombinase to DJH Junctions.” Nature immunology 13 (12): 1205-1212. doi:10.1038/ni.2447. http://dx.doi.org/10.1038/ni.2447.
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Abstract: Immunoglobulin heavy chain (Igh) genes are assembled by sequential rearrangements of diversity (DH) and variable (VH) gene segments. Three critical constraints govern VH recombination. These include timing (VH recombination follows DH recombination), precision (VHs recombine only to DJH junctions) and allele specificity (VH recombination is restricted to DJH recombined alleles). We provide a model for these universal features of VH recombination. Analyses of DJH recombined alleles revealed that DJH junctions were selectively epigenetically marked, became nuclease sensitive and bound RAG proteins, thereby permitting DH-associated recombination signal sequences to initiate the second step of Igh gene assembly. We propose that VH recombination is precise because these changes did not extend to germline DH gene segments located 5′ of the DJH junction.
Published Version: doi:10.1038/ni.2447
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685187/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11708617
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