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dc.contributor.authorSubrahmanyam, Rameshen_US
dc.contributor.authorDu, Hansenen_US
dc.contributor.authorIvanova, Irinaen_US
dc.contributor.authorChakraborty, Tirthaen_US
dc.contributor.authorJi, Yanhongen_US
dc.contributor.authorZhang, Yuen_US
dc.contributor.authorAlt, Frederick W.en_US
dc.contributor.authorSchatz, David G.en_US
dc.contributor.authorSen, Ranjanen_US
dc.date.accessioned2014-02-13T19:01:46Z
dc.date.issued2013en_US
dc.identifier.citationSubrahmanyam, Ramesh, Hansen Du, Irina Ivanova, Tirtha Chakraborty, Yanhong Ji, Yu Zhang, Frederick W. Alt, David G. Schatz, and Ranjan Sen. 2013. “Localized Epigenetic Changes Induced by DH Recombination Restricts Recombinase to DJH Junctions.” Nature immunology 13 (12): 1205-1212. doi:10.1038/ni.2447. http://dx.doi.org/10.1038/ni.2447.en
dc.identifier.issn1529-2908en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11708617
dc.description.abstractImmunoglobulin heavy chain (Igh) genes are assembled by sequential rearrangements of diversity (DH) and variable (VH) gene segments. Three critical constraints govern VH recombination. These include timing (VH recombination follows DH recombination), precision (VHs recombine only to DJH junctions) and allele specificity (VH recombination is restricted to DJH recombined alleles). We provide a model for these universal features of VH recombination. Analyses of DJH recombined alleles revealed that DJH junctions were selectively epigenetically marked, became nuclease sensitive and bound RAG proteins, thereby permitting DH-associated recombination signal sequences to initiate the second step of Igh gene assembly. We propose that VH recombination is precise because these changes did not extend to germline DH gene segments located 5′ of the DJH junction.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/ni.2447en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685187/pdf/en
dash.licenseLAAen_US
dc.titleLocalized Epigenetic Changes Induced by DH Recombination Restricts Recombinase to DJH Junctionsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature immunologyen
dash.depositing.authorZhang, Yuen_US
dc.date.available2014-02-13T19:01:46Z
dc.identifier.doi10.1038/ni.2447*
dash.contributor.affiliatedZhang, Yu
dash.contributor.affiliatedAlt, Frederick


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