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dc.contributor.authorJeselsohn, Rinath M.en_US
dc.contributor.authorWerner, Lillianen_US
dc.contributor.authorRegan, Meredith M.en_US
dc.contributor.authorFatima, Aquilaen_US
dc.contributor.authorGilmore, Laurenen_US
dc.contributor.authorCollins, Laura C.en_US
dc.contributor.authorBeck, Andrew H.en_US
dc.contributor.authorBailey, Shannon T.en_US
dc.contributor.authorHe, Housheng Hansenen_US
dc.contributor.authorBuchwalter, Gillesen_US
dc.contributor.authorBrown, Mylesen_US
dc.contributor.authorIglehart, J. Dirken_US
dc.contributor.authorRichardson, Andreaen_US
dc.contributor.authorCome, Steven E.en_US
dc.date.accessioned2014-02-13T19:01:59Z
dc.date.issued2013en_US
dc.identifier.citationJeselsohn, R. M., L. Werner, M. M. Regan, A. Fatima, L. Gilmore, L. C. Collins, A. H. Beck, et al. 2013. “Digital Quantification of Gene Expression in Sequential Breast Cancer Biopsies Reveals Activation of an Immune Response.” PLoS ONE 8 (5): e64225. doi:10.1371/journal.pone.0064225. http://dx.doi.org/10.1371/journal.pone.0064225.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11708657
dc.description.abstractAdvancements in molecular biology have unveiled multiple breast cancer promoting pathways and potential therapeutic targets. Large randomized clinical trials remain the ultimate means of validating therapeutic efficacy, but they require large cohorts of patients and are lengthy and costly. A useful approach is to conduct a window of opportunity study in which patients are exposed to a drug pre-surgically during the interval between the core needle biopsy and the definitive surgery. These are non-therapeutic studies and the end point is not clinical or pathological response but rather evaluation of molecular changes in the tumor specimens that can predict response. However, since the end points of the non-therapeutic studies are biologic, it is critical to first define the biologic changes that occur in the absence of treatment. In this study, we compared the molecular profiles of breast cancer tumors at the time of the diagnostic biopsy versus the definitive surgery in the absence of any intervention using the Nanostring nCounter platform. We found that while the majority of the transcripts did not vary between the two biopsies, there was evidence of activation of immune related genes in response to the first biopsy and further investigations of the immune changes after a biopsy in early breast cancer seem warranted.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0064225en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669373/pdf/en
dash.licenseLAAen_US
dc.subjectBiologyen
dc.subjectComputational Biologyen
dc.subjectMolecular Geneticsen
dc.subjectGene Expressionen
dc.subjectGeneticsen
dc.subjectHuman Geneticsen
dc.subjectGenetic Testingen
dc.subjectCancer Geneticsen
dc.subjectGenetic Screensen
dc.subjectGenetics of Diseaseen
dc.subjectGenomicsen
dc.subjectGenome Analysis Toolsen
dc.subjectGenome Expression Analysisen
dc.subjectGenomic Medicineen
dc.subjectMolecular Cell Biologyen
dc.subjectMedicineen
dc.subjectOncologyen
dc.subjectCancers and Neoplasmsen
dc.subjectBreast Tumorsen
dc.subjectInvasive Ductal Carcinomaen
dc.titleDigital Quantification of Gene Expression in Sequential Breast Cancer Biopsies Reveals Activation of an Immune Responseen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorJeselsohn, Rinath M.en_US
dc.date.available2014-02-13T19:01:59Z
dc.identifier.doi10.1371/journal.pone.0064225*
dash.authorsorderedfalse
dash.contributor.affiliatedBailey, Shannon T.
dash.contributor.affiliatedBuchwalter, Gilles
dash.contributor.affiliatedGilmore, Lauren
dash.contributor.affiliatedCome, Steven
dash.contributor.affiliatedJeselsohn, Rinath
dash.contributor.affiliatedCollins, Laura
dash.contributor.affiliatedBeck, Andrew
dash.contributor.affiliatedRichardson, Andrea
dash.contributor.affiliatedBrown, Myles
dash.contributor.affiliatedRegan, Meredith


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