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dc.contributor.authorAllard, Patricken_US
dc.contributor.authorKleinstreuer, Nicole C.en_US
dc.contributor.authorKnudsen, Thomas B.en_US
dc.contributor.authorColaiácovo, Monica P.en_US
dc.date.accessioned2014-02-13T19:02:02Z
dc.date.issued2013en_US
dc.identifier.citationAllard, Patrick, Nicole C. Kleinstreuer, Thomas B. Knudsen, and Monica P. Colaiácovo. 2013. “A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function.” Environmental Health Perspectives 121 (6): 717-724. doi:10.1289/ehp.1206301. http://dx.doi.org/10.1289/ehp.1206301.en
dc.identifier.issn0091-6765en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11708664
dc.description.abstractBackground: Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals. Objectives: We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure. Methods: We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database. Results: The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression. Conclusions: This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function.en
dc.language.isoen_USen
dc.publisherNational Institute of Environmental Health Sciencesen
dc.relation.isversionofdoi:10.1289/ehp.1206301en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672921/pdf/en
dash.licenseLAAen_US
dc.subjectaneuploidyen
dc.subjecten
dc.subjectchromosome segregationen
dc.subjectgermlineen
dc.subjectpesticidesen
dc.titleA C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Functionen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalEnvironmental Health Perspectivesen
dash.depositing.authorColaiácovo, Monica P.en_US
dc.date.available2014-02-13T19:02:02Z
dc.identifier.doi10.1289/ehp.1206301*
dash.contributor.affiliatedColaiacovo, Monica


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