Peptidomic discovery of short open reading frame-encoded peptides in human cells
Mitchell, Andrew J.
Schwaid, Adam G.
Cabili, Moran N.
Budnik, Bogdan A.
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CitationSlavoff, Sarah A., Andrew J. Mitchell, Adam G. Schwaid, Moran N. Cabili, Jiao Ma, Joshua Z. Levin, Amir D. Karger, Bogdan A. Budnik, John L. Rinn, and Alan Saghatelian. 2013. “Peptidomic discovery of short open reading frame-encoded peptides in human cells.” Nature chemical biology 9 (1): 59-64. doi:10.1038/nchembio.1120. http://dx.doi.org/10.1038/nchembio.1120.
AbstractThe amount of the transcriptome that is translated into polypeptides is of fundamental importance. We developed a peptidomic strategy to detect short ORF (sORF)-encoded polypeptides (SEPs) in human cells. We identified 90 SEPs, 86 of which are novel, the largest number of human SEPs ever reported. SEP abundances range from 10-1000 molecules per cell, identical to known proteins. SEPs arise from sORFs in non-coding RNAs as well as multi-cistronic mRNAs, and many SEPs initiate with non-AUG start codons, indicating that non-canonical translation may be more widespread in mammals than previously thought. In addition, coding sORFs are present in a small fraction (8/1866) of long intergenic non-coding RNAs (lincRNAs). Together, these results provide the strongest evidence to date that the human proteome is more complex than previously appreciated.
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