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dc.contributor.authorSlavoff, Sarah A.en_US
dc.contributor.authorMitchell, Andrew J.en_US
dc.contributor.authorSchwaid, Adam G.en_US
dc.contributor.authorCabili, Moran N.en_US
dc.contributor.authorMa, Jiaoen_US
dc.contributor.authorLevin, Joshua Z.en_US
dc.contributor.authorKarger, Amiren_US
dc.contributor.authorBudnik, Bogdan A.en_US
dc.contributor.authorRinn, John L.en_US
dc.contributor.authorSaghatelian, Alanen_US
dc.date.accessioned2014-02-18T18:10:27Z
dc.date.issued2013en_US
dc.identifier.citationSlavoff, Sarah A., Andrew J. Mitchell, Adam G. Schwaid, Moran N. Cabili, Jiao Ma, Joshua Z. Levin, Amir D. Karger, Bogdan A. Budnik, John L. Rinn, and Alan Saghatelian. 2013. “Peptidomic discovery of short open reading frame-encoded peptides in human cells.” Nature chemical biology 9 (1): 59-64. doi:10.1038/nchembio.1120. http://dx.doi.org/10.1038/nchembio.1120.en
dc.identifier.issn1552-4450en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11717493
dc.description.abstractThe amount of the transcriptome that is translated into polypeptides is of fundamental importance. We developed a peptidomic strategy to detect short ORF (sORF)-encoded polypeptides (SEPs) in human cells. We identified 90 SEPs, 86 of which are novel, the largest number of human SEPs ever reported. SEP abundances range from 10-1000 molecules per cell, identical to known proteins. SEPs arise from sORFs in non-coding RNAs as well as multi-cistronic mRNAs, and many SEPs initiate with non-AUG start codons, indicating that non-canonical translation may be more widespread in mammals than previously thought. In addition, coding sORFs are present in a small fraction (8/1866) of long intergenic non-coding RNAs (lincRNAs). Together, these results provide the strongest evidence to date that the human proteome is more complex than previously appreciated.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nchembio.1120en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625679/pdf/en
dash.licenseLAAen_US
dc.titlePeptidomic discovery of short open reading frame-encoded peptides in human cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature chemical biologyen
dash.depositing.authorSlavoff, Sarah A.en_US
dc.date.available2014-02-18T18:10:27Z
dc.identifier.doi10.1038/nchembio.1120*
dash.contributor.affiliatedSlavoff, Sarah A.
dash.contributor.affiliatedMa, Jiao
dash.contributor.affiliatedSaghatelian, Alan
dash.contributor.affiliatedKarger, Amir
dash.contributor.affiliatedLevin, Joshua Z.
dash.contributor.affiliatedRinn, John


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