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dc.contributor.authorKatz, Lee S.en_US
dc.contributor.authorPetkau, Aaronen_US
dc.contributor.authorBeaulaurier, Johnen_US
dc.contributor.authorTyler, Shaunen_US
dc.contributor.authorAntonova, Elena S.en_US
dc.contributor.authorTurnsek, Maryann A.en_US
dc.contributor.authorGuo, Yanen_US
dc.contributor.authorWang, Susanaen_US
dc.contributor.authorPaxinos, Ellen E.en_US
dc.contributor.authorOrata, Fabinien_US
dc.contributor.authorGladney, Lori M.en_US
dc.contributor.authorStroika, Stevenen_US
dc.contributor.authorFolster, Jason P.en_US
dc.contributor.authorRowe, Lorien_US
dc.contributor.authorFreeman, Molly M.en_US
dc.contributor.authorKnox, Natalieen_US
dc.contributor.authorFrace, Mikeen_US
dc.contributor.authorBoncy, Jacquesen_US
dc.contributor.authorGraham, Moragen_US
dc.contributor.authorHammer, Brian K.en_US
dc.contributor.authorBoucher, Yanen_US
dc.contributor.authorBashir, Alien_US
dc.contributor.authorHanage, William P.en_US
dc.contributor.authorVan Domselaar, Garyen_US
dc.contributor.authorTarr, Cheryl L.en_US
dc.date.accessioned2014-02-18T18:10:57Z
dc.date.issued2013en_US
dc.identifier.citationKatz, L. S., A. Petkau, J. Beaulaurier, S. Tyler, E. S. Antonova, M. A. Turnsek, Y. Guo, et al. 2013. “Evolutionary Dynamics of Vibrio cholerae O1 following a Single-Source Introduction to Haiti.” mBio 4 (4): e00398-13. doi:10.1128/mBio.00398-13. http://dx.doi.org/10.1128/mBio.00398-13.en
dc.identifier.issn2150-7511en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11717532
dc.description.abstractABSTRACT Prior to the epidemic that emerged in Haiti in October of 2010, cholera had not been documented in this country. After its introduction, a strain of Vibrio cholerae O1 spread rapidly throughout Haiti, where it caused over 600,000 cases of disease and >7,500 deaths in the first two years of the epidemic. We applied whole-genome sequencing to a temporal series of V. cholerae isolates from Haiti to gain insight into the mode and tempo of evolution in this isolated population of V. cholerae O1. Phylogenetic and Bayesian analyses supported the hypothesis that all isolates in the sample set diverged from a common ancestor within a time frame that is consistent with epidemiological observations. A pangenome analysis showed nearly homogeneous genomic content, with no evidence of gene acquisition among Haiti isolates. Nine nearly closed genomes assembled from continuous-long-read data showed evidence of genome rearrangements and supported the observation of no gene acquisition among isolates. Thus, intrinsic mutational processes can account for virtually all of the observed genetic polymorphism, with no demonstrable contribution from horizontal gene transfer (HGT). Consistent with this, the 12 Haiti isolates tested by laboratory HGT assays were severely impaired for transformation, although unlike previously characterized noncompetent V. cholerae isolates, each expressed hapR and possessed a functional quorum-sensing system. Continued monitoring of V. cholerae in Haiti will illuminate the processes influencing the origin and fate of genome variants, which will facilitate interpretation of genetic variation in future epidemics.en
dc.language.isoen_USen
dc.publisherAmerican Society of Microbiologyen
dc.relation.isversionofdoi:10.1128/mBio.00398-13en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705451/pdf/en
dash.licenseLAAen_US
dc.titleEvolutionary Dynamics of Vibrio cholerae O1 following a Single-Source Introduction to Haitien
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalmBioen
dash.depositing.authorHanage, William P.en_US
dc.date.available2014-02-18T18:10:57Z
dc.identifier.doi10.1128/mBio.00398-13*
dash.authorsorderedfalse
dash.contributor.affiliatedHanage, William


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