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dc.contributor.authorUsta, O. Berken_US
dc.contributor.authorKim, Yeonheeen_US
dc.contributor.authorOzer, Sinanen_US
dc.contributor.authorBruinsma, Bote G.en_US
dc.contributor.authorLee, Jungwooen_US
dc.contributor.authorDemir, Esinen_US
dc.contributor.authorBerendsen, Tim A.en_US
dc.contributor.authorPuts, Catheleyne F.en_US
dc.contributor.authorIzamis, Maria-Louisaen_US
dc.contributor.authorUygun, Korkuten_US
dc.contributor.authorUygun, Basak E.en_US
dc.contributor.authorYarmush, Martin L.en_US
dc.date.accessioned2014-02-18T18:11:38Z
dc.date.issued2013en_US
dc.identifier.citationUsta, O. B., Y. Kim, S. Ozer, B. G. Bruinsma, J. Lee, E. Demir, T. A. Berendsen, et al. 2013. “Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes.” PLoS ONE 8 (7): e69334. doi:10.1371/journal.pone.0069334. http://dx.doi.org/10.1371/journal.pone.0069334.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11717595
dc.description.abstractSupercooling preservation holds the potential to drastically extend the preservation time of organs, tissues and engineered tissue products, and fragile cell types that do not lend themselves well to cryopreservation or vitrification. Here, we investigate the effects of supercooling preservation (SCP at -4oC) on primary rat hepatocytes stored in cryovials and compare its success (high viability and good functional characteristics) to that of static cold storage (CS at +4oC) and cryopreservation. We consider two prominent preservation solutions a) Hypothermosol (HTS-FRS) and b) University of Wisconsin solution (UW) and a range of preservation temperatures (-4 to -10 oC). We find that there exists an optimum temperature (-4oC) for SCP of rat hepatocytes which yields the highest viability; at this temperature HTS-FRS significantly outperforms UW solution in terms of viability and functional characteristics (secretions and enzymatic activity in suspension and plate culture). With the HTS-FRS solution we show that the cells can be stored for up to a week with high viability (~56%); moreover we also show that the preservation can be performed in large batches (50 million cells) with equal or better viability and no loss of functionality as compared to smaller batches (1.5 million cells) performed in cryovials.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0069334en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713052/pdf/en
dash.licenseLAAen_US
dc.titleSupercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorBruinsma, Bote G.en_US
dc.date.available2014-02-18T18:11:38Z
dc.identifier.doi10.1371/journal.pone.0069334*
dash.authorsorderedfalse
dash.contributor.affiliatedLee, Jungwoo
dash.contributor.affiliatedBruinsma, Bote G.
dash.contributor.affiliatedUygun, Basak
dash.contributor.affiliatedYarmush, Martin


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