A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

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Author
Zou, Lijin
Luo, Yonglun
Chen, Muwan
Ding, Ming
Petersen, Charlotte Christie
Kang, Ran
Dagnaes-Hansen, Frederik
Zeng, Yuanlin
Lv, Nonghua
Ma, Qing
Le, Dang Q. S.
Besenbacher, Flemming
Bolund, Lars
Jensen, Thomas G.
Kjems, Jørgen
Bünger, Cody
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1038/srep02243Metadata
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Zou, L., Y. Luo, M. Chen, G. Wang, M. Ding, C. C. Petersen, R. Kang, et al. 2013. “A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds.” Scientific Reports 3 (1): 2243. doi:10.1038/srep02243. http://dx.doi.org/10.1038/srep02243.Abstract
We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency – as seen with the loss of markers OCT3/4 and TRA-1-81 – and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718204/pdf/Terms of Use
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