Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma

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Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma

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Title: Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma
Author: RIGHI, VALERIA; ANDRONESI, OVIDIU; MINTZOPOULOS, DIONYSSIOS; TZIKA, A. ARIA

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Citation: RIGHI, VALERIA, OVIDIU ANDRONESI, DIONYSSIOS MINTZOPOULOS, and A. ARIA TZIKA. 2009. “Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma.” International Journal of Molecular Medicine 24 (6): 749-757. doi:10.3892/ijmm_00000288. http://dx.doi.org/10.3892/ijmm_00000288.
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Abstract: We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D)1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40–60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state analogue TOCSY (TOtal Correlation SpectroscopY). Using 1-and 2-dimensional HRMAS NMR experiments, we identified several metabolites in burned tissues. Quantification of metabolites in burned tissues showed increased levels of lipid compounds, intracellular metabolites (e.g., taurine and phosphocreatine) and substantially decreased water-soluble metabolites (e.g., glutathione, carnosine, glucose, glutamine/glutamate and alanine). These findings demonstrate that HRMAS NMR Spectroscopy using TOBSY is a feasible technique that reveals new insights into the pathophysiology of burn trauma. Moreover, this method has applications that facilitate the development of novel therapeutic strategies.
Published Version: doi:10.3892/ijmm_00000288
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722686/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11717627
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