Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns

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Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns

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Title: Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns
Author: Bose, Suman; Singh, Rishi; Hanewich-Hollatz, Mikhail; Shen, Chong; Lee, Chia-Hua; Dorfman, David M.; Karp, Jeffrey M.; Karnik, Rohit

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Citation: Bose, Suman, Rishi Singh, Mikhail Hanewich-Hollatz, Chong Shen, Chia-Hua Lee, David M. Dorfman, Jeffrey M. Karp, and Rohit Karnik. 2013. “Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns.” Scientific Reports 3 (1): 2329. doi:10.1038/srep02329. http://dx.doi.org/10.1038/srep02329.
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Abstract: Flow fractionation of cells using physical fields to achieve lateral displacement finds wide applications, but its extension to surface molecule-specific separation requires labeling. Here we demonstrate affinity flow fractionation (AFF) where weak, short-range interactions with asymmetric molecular patterns laterally displace cells in a continuous, label-free process. We show that AFF can directly draw neutrophils out of a continuously flowing stream of blood with an unprecedented 400,000-fold depletion of red blood cells, with the sorted cells being highly viable, unactivated, and functionally intact. The lack of background erythrocytes enabled the use of AFF for direct enumeration of neutrophils by a downstream detector, which could distinguish the activation state of neutrophils in blood. The compatibility of AFF with capillary microfluidics and its ability to directly separate cells with high purity and minimal sample preparation will facilitate the design of simple and portable devices for point-of-care diagnostics and quick, cost-effective laboratory analysis.
Published Version: doi:10.1038/srep02329
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728592/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11717635
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