Engineered ascorbate peroxidase as a genetically-encoded reporter for electron microscopy
Martell, Jeffrey D.
Deerinck, Thomas J.
Poulos, Thomas L.
Sosinsky, Gina E.
Ellisman, Mark H.
Ting, Alice Y.
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CitationMartell, Jeffrey D., Thomas J. Deerinck, Yasemin Sancak, Thomas L. Poulos, Vamsi K. Mootha, Gina E. Sosinsky, Mark H. Ellisman, and Alice Y. Ting. 2013. “Engineered ascorbate peroxidase as a genetically-encoded reporter for electron microscopy.” Nature biotechnology 30 (11): 1143-1148. doi:10.1038/nbt.2375. http://dx.doi.org/10.1038/nbt.2375.
AbstractElectron microscopy (EM) is the standard method for imaging cellular structures with nanometer resolution, but existing genetic tags are inactive in most cellular compartments1 or require light and are difficult to use2. Here we report the development of a simple and robust EM genetic tag, called “APEX,” that is active in all cellular compartments and does not require light. APEX is a monomeric 28 kDa peroxidase that withstands strong EM fixation to give excellent ultrastructural preservation. We demonstrate the utility of APEX for high-resolution EM imaging of a variety of mammalian organelles and specific proteins. We also fused APEX to the N- or C-terminus of the mitochondrial calcium uniporter (MCU), a newly identified channel whose topology is disputed3,4. MCU-APEX and APEX-MCU give EM contrast exclusively in the mitochondrial matrix, suggesting that both the N-and C-termini of MCU face the matrix.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11717661
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