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dc.contributor.authorMartell, Jeffrey D.en_US
dc.contributor.authorDeerinck, Thomas J.en_US
dc.contributor.authorSancak, Yaseminen_US
dc.contributor.authorPoulos, Thomas L.en_US
dc.contributor.authorMootha, Vamsi K.en_US
dc.contributor.authorSosinsky, Gina E.en_US
dc.contributor.authorEllisman, Mark H.en_US
dc.contributor.authorTing, Alice Y.en_US
dc.date.accessioned2014-02-18T18:11:59Z
dc.date.issued2013en_US
dc.identifier.citationMartell, Jeffrey D., Thomas J. Deerinck, Yasemin Sancak, Thomas L. Poulos, Vamsi K. Mootha, Gina E. Sosinsky, Mark H. Ellisman, and Alice Y. Ting. 2013. “Engineered ascorbate peroxidase as a genetically-encoded reporter for electron microscopy.” Nature biotechnology 30 (11): 1143-1148. doi:10.1038/nbt.2375. http://dx.doi.org/10.1038/nbt.2375.en
dc.identifier.issn1087-0156en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11717661
dc.description.abstractElectron microscopy (EM) is the standard method for imaging cellular structures with nanometer resolution, but existing genetic tags are inactive in most cellular compartments1 or require light and are difficult to use2. Here we report the development of a simple and robust EM genetic tag, called “APEX,” that is active in all cellular compartments and does not require light. APEX is a monomeric 28 kDa peroxidase that withstands strong EM fixation to give excellent ultrastructural preservation. We demonstrate the utility of APEX for high-resolution EM imaging of a variety of mammalian organelles and specific proteins. We also fused APEX to the N- or C-terminus of the mitochondrial calcium uniporter (MCU), a newly identified channel whose topology is disputed3,4. MCU-APEX and APEX-MCU give EM contrast exclusively in the mitochondrial matrix, suggesting that both the N-and C-termini of MCU face the matrix.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nbt.2375en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699407/pdf/en
dash.licenseLAAen_US
dc.titleEngineered ascorbate peroxidase as a genetically-encoded reporter for electron microscopyen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature biotechnologyen
dash.depositing.authorSancak, Yaseminen_US
dc.date.available2014-02-18T18:11:59Z
dc.identifier.doi10.1038/nbt.2375*
dash.contributor.affiliatedSancak, Yasemin
dash.contributor.affiliatedMootha, Vamsi


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