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dc.contributor.authorWu, Jun
dc.contributor.authorShekhar, Nandini
dc.contributor.authorLele, Pushkar Prakash
dc.contributor.authorLele, Tanmay P.
dc.date.accessioned2014-02-20T21:22:21Z
dc.date.issued2012
dc.identifier.citationWu, Jun, Nandini Shekhar, Pushkar P. Lele, and Tanmay P. Lele. 2012. Frap analysis: accounting for bleaching during image capture. PLoS ONE 7(8): e42854.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11726260
dc.description.abstractThe analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0042854en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415426/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectProteinsen_US
dc.subjectProtein Chemistryen_US
dc.subjectBiophysicsen_US
dc.subjectBiophysics Theoryen_US
dc.subjectMacromolecular Assembliesen_US
dc.subjectDevelopmental Biologyen_US
dc.subjectMolecular Developmenten_US
dc.subjectAdhesion Moleculesen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCell Adhesionen_US
dc.subjectSystems Biologyen_US
dc.subjectChemistryen_US
dc.subjectPhysical Chemistryen_US
dc.subjectChemical Dynamicsen_US
dc.subjectEngineeringen_US
dc.subjectBioengineeringen_US
dc.subjectBiological Systems Engineeringen_US
dc.subjectPhysicsen_US
dc.titleFRAP Analysis: Accounting for Bleaching during Image Captureen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorLele, Pushkar Prakash
dc.date.available2014-02-20T21:22:21Z
dc.identifier.doi10.1371/journal.pone.0042854*
dash.authorsorderedfalse
dash.contributor.affiliatedLele, Pushkar Prakash


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