Mimicking p14ARF Phosphorylation Influences Its Ability to Restrain Cell Proliferation

DSpace/Manakin Repository

Mimicking p14ARF Phosphorylation Influences Its Ability to Restrain Cell Proliferation

Citable link to this page

 

 
Title: Mimicking p14ARF Phosphorylation Influences Its Ability to Restrain Cell Proliferation
Author: Vivo, Maria; Ranieri, Michela; Sansone, Federica; Santoriello, Cristina; Calogero, Raffaele A.; Calabrò, Viola; Pollice, Alessandra; La Mantia, Girolama

Note: Order does not necessarily reflect citation order of authors.

Citation: Vivo, Maria, Michela Ranieri, Federica Sansone, Cristina Santoriello, Raffaele A. Calogero, Viola Calabrò, Alessandra Pollice, and Girolama La Mantia. 2013. Mimicking p14arf phosphorylation influences its ability to restrain cell proliferation. PLoS ONE 8(1): e53631.
Full Text & Related Files:
Abstract: The INK4a/ARF locus on the short arm of chromosome 9 is one of the most frequently altered loci in human cancer. It is generally accepted that ARF is involved in oncogenic checkpoint pathways by sensitizing incipient cancer cells to undergo growth arrest or apoptosis through both p53-dependent and independent pathways. While intensive studies have been focused on ARF activation at the transcriptional level, only recently mechanisms governing ARF turnover have been identified. Here, we show for the first time that p14ARF is a PKC target. Prediction analysis showed many potential phosphorylation sites in PKC consensus sequences within ARF protein, and, among them, the threonine at position 8 was the most conserved. Substitution of this threonine influences both ARF stability and localization. Furthermore, a phosphomimetic ARF mutation reduces the ability to arrest cell growth although the ability to bind MDM2 and stabilize p53 result unaffected. Thus we propose that phosphorylation of ARF in both immortalized and tumor cell lines could be a mechanism to escape ARF surveillance following proliferative and oncogenic stress.
Published Version: doi:10.1371/journal.pone.0053631
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538741/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11729521
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters