DNA Ligase-Mediated Translation of DNA Into Densely Functionalized Nucleic Acid Polymers

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DNA Ligase-Mediated Translation of DNA Into Densely Functionalized Nucleic Acid Polymers

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Title: DNA Ligase-Mediated Translation of DNA Into Densely Functionalized Nucleic Acid Polymers
Author: Hili, Ryan; Niu, Jia; Liu, David Ruchien

Note: Order does not necessarily reflect citation order of authors.

Citation: Hili, Ryan, Jia Niu, and David R. Liu. 2012. DNA ligase-mediated translation of dna into densely functionalized nucleic acid polymers. Journal of the American Chemical Society 135(1): 98-101.
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Abstract: We developed a method to translate DNA sequences into densely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerization of 5′-phosphorylated trinucleotides containing a wide variety of appended functional groups. This polymerization proceeds sequence specifically along a DNA template and can generate polymers of at least 50 building blocks (150 nucleotides) in length with remarkable efficiency. The resulting single-stranded highly modified nucleic acid is a suitable template for primer extension using deep vent (exo-) DNA polymerase, thereby enabling the regeneration of template DNA. We integrated these capabilities to perform iterated cycles of in vitro translation, selection, and template regeneration on libraries of modified nucleic acid polymers.
Published Version: doi:10.1021/ja311331m
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544274/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11729523
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