Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling

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Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling

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Title: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling
Author: Boyle, Patrick; Clement, Kendell; Gu, Hongcang; Smith, Zachary D; Ziller, Michael Johannes; Fostel, Jennifer L; Holmes, Laurie; Meldrim, Jim; Kelley, Fontina; Gnirke, Andreas; Meissner, Alexander

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Citation: Boyle, Patrick, Kendell Clement, Hongcang Gu, Zachary D Smith, Michael Ziller, Jennifer L Fostel, Laurie Holmes, et al. 2012. Gel-free multiplexed reduced representation bisulfite sequencing for large-scale dna methylation profiling. Genome Biology 13(10): R92.
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Abstract: Sequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage to the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies, including cohorts of cancer samples.
Published Version: doi:10.1186/gb-2012-13-10-r92
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491420/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11729537
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